1. 【代表性成果】

蜂王浆高产机理及蜂王浆生化特征的研究，获得北京市科学技术奖，二等奖，2014年。

2. 【代表性论文清单】

1.Han B, Wei QH, Wu F, Hu H, Ma C, Meng LF, Zhang XF, Feng M, Fang Y, Rueppell O, Li J. Tachykinin signaling inhibits task-specific behavioral responsiveness in honeybee workers. Elife. 2021. 10:e64830.

2. Zhang XF, Hu H, Han B, Wei QH, Meng LF, Wu F, Fang Y, Feng M, Ma C, Rueppell O, Li J: The neuroproteomic basis of enhanced perception and processing of brood signals that trigger increased reproductive investment in honeybee (Apis mellifera) workers. Molecular & Cellular Proteomics 2020, 19(10):1632-1648.

3. Han Hu, Gebreamlak Bezabih, Mao Feng, Qiaohong Wei, Xufeng Zhang, Fan Wu, Lifeng Meng, Yu Fang, Bin Han, Chuan Ma, Jianke Li. In-depth proteome of the hypopharygeal glands of honeybee workers reveals highly activated protein and energy metabolism in priming the secretion of royal jelly. Molecular & Cellular Proteomics, 2019, 18: 606-621.

4. Meng L ,Huo X ,Mao F , et al. Proteomics Reveals the Molecular Underpinnings of Stronger Learning and Memory in Eastern Compared to Western Bees. Molecular & Cellular Proteomics Mcp, 2018, 17(2):255-269.

5. Fang Y ,Feng M ,Han B , et al. In-depth Proteomics Characterization of Embryogenesis of the Honey Bee Worker (Apis mellifera L.). Molecular & Cellular Proteomics. 2014. 13(9).

6. Fan Wu, Chuan Ma, Bin Han, Lifeng Meng, Han Hu, Yu Fang, Mao Feng, Xufeng Zhang, Olav Rueppell, Jianke Li. Behavioral, physiological, and molecular changes in alloparental care givers may be responsible for selection response for female reproductive investment in honey bees. Molecular Ecology, 2019, 28(18): 4212-4227.

7. Meng LF, Altaye SZ, Feng M, Hu H, Han B, Li JK. Phosphoproteomic basis of neuroplasticity in the antennal lobes influences the olfactory differences between A. mellifera and A. cerana honeybees. Journal of Proteomics. 2022. 251, e104413.

8.Hu H, Wei QH, Sun ZH, Zhang XF, Ma C, Feng M, Meng LF, Li JK, Han B. Development of a freshness assay for royal jelly based on the temperature- and time-dependent changes of antimicrobial effectiveness and proteome dynamics of royal jelly proteins. Journal of Agricultural and Food Chemistry. 2021. 69(36):10731-10740.

9. Feng M, Fang Y, Ma C, Duan XY, Zhang YY, Han B, Hu H, Meng LF, Wang FY, Li JK. Mechanistic insight into royal protein inhibiting the Gram-positive bacteria. Biomolecules. 2021.11(1):e64.

10. Ma C, Zhang L, Feng M, Fang Y, Hu H, Han B, Meng L, Li J. Metabolic profiling unravels the effects of enhanced output and harvesting time on royal jelly quality. Food Research International. 2021. 139:e109974.

11. Ma C, Li J. Characterization of the mitochondrial genome of a high royal jelly-producing honeybee strain (Apis mellifera ligustica). Mitochondrial DNA Part B-Resources. 2021.6(10):2939-2940.

12. Ahmad S, Khan SA, Khan KA, Li JK. Novel insight into the development and function of hypopharyngeal glands in honey bees. Frontiers in Physiology. 2021. 11:e615830.

13. Fan P, Han B, Hu H, Wei QH, Zhang XF, Meng LF, Nie J, Tang XF, Tian XY, Zhang L, Wang L, Li J: Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice. Expert Opinion on Therapeutic Targets. 2020.24(3):267-279.

14. Qi D, Ma C, Wang W, Zhang L, Hao J, Li J: Gas chromatography-mass spectrometry analysis reveals the differences in volatile components of royal jelly from different honeybee stocks. LWT-Food Science and Technology. 2020.124: e109143.

15. Ahmad S, Campos MG, Fratini F, Altaye SZ, Li JK: New insights into the biological and pharmaceutical properties of royal jelly. International Journal of Molecular Sciences. 2020. 21(2).

16. Qi DD, Ma CY, Wang WW, Zhang LC, Li JK: Gas chromatography-mass spectrometry analysis as a tool to reveal differences between the volatile compound profile of royal jelly produced from tea and pagoda trees. Food Analytical Methods. 2020.

17. Wu J, Elsheikha H M, Tu Y , et al. Significant transcriptional changes in mature daughter Varroa destructor mites during infestation of different developmental stages of honeybees. Pest Management Science. 2020.

18. Getachew A, TAAbejew,Wu J , et al. Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen. Scientific Reports. 10.1038/s41598-020-64022-3

19.Wu Jiangli, Hany M Elsheikha, Yangyang Tu, Awraris Getachew,Huaiyu Zhou, Chunxue Zhou, Shufa Xu. Significant transcriptional changes in mature daughter Varroa destructor mites during infestation of different developmental stages of honeybees. Pest Management science, 2020.

20. Solomon Zewdu Altaye, Lifeng Meng, Jianke Li. Molecular insights into the enhanced performance of royal jelly secretion by a stock of honeybee (Apis mellifera ligustica) selected for increasing royal jelly production. Apidologie, 2019. 1-18.

21. Solomon Zewdu Altaye, Lifeng Meng, Yao Lu, Jianke Li. The emerging proteomic research facilitates in-depth understanding of the biology of honeybees. International Journal of Molecular Sciences. 2019. 20(17): 4252.

22. Fan Wu, Yilu Feng, Bin Han, Han Hu, Mao Feng, Lifeng Meng, Chuan Ma, Linsheng Yu, Jianke Li. Mechanistic insight into binding interaction between chemosensory protein 4 and volatile larval pheromones in honeybees (Apis mellifera). International Journal of Biological Macromolecules. 2019. 141: 553-563.

23.Chuan Ma, Licui Zhang, Jianke Li. The complete mitochondrial genome of a field cricket Turanogryllus eous (Insecta: Orthoptera). Mitochondrial DNA Part B. 2019. 4(2): 3852-3853.

24.Chuan Ma, Yeying Wang, Licui Zhang, Jianke Li. Mitochondrial genome characterization of the family Trigonidiidae (Orthoptera) reveals novel structural features and nad1 transcript ends. Scientific Reports. 2019. 9(1):19092.

25. Ararso Z, Ma C, Qi Y, Feng M, Han B, Hu H, Meng L, Li J. Proteome Comparisons between Hemolymph of Two Honeybee Strains (Apis mellifera ligustica) Reveal Divergent Molecular Basis in Driving Hemolymph Function and High Royal Jelly Secretion. Journal of Proteome Research. 2018. 17, 402−419. (IF 4.1)

26. Hora ZA, Altaye SZ, Wubie AJ, Li J. Proteomics Improves the New Understanding of Honeybee Biology, J Agric Food Chem.2018. 66(14):3605-3615. (IF 3.4)

27. Ma C, Li J. Comparative analysis of mitochondrial genomes of the superfamily Grylloidea (Insecta, Orthoptera) reveals phylogenetic distribution of gene rearrangements. Int J Biol Macromol. 2018. 120 (Pt A):1048-1054. (IF 3.9)

28. Huo XM, Meng LF, Jiang T, Li M, Sun FZ , Sun B, Li JK. Real-time observation of nucleoplasmin-mediated DNA decondensation and condensation reveals its specific functions as a chaperone. Biochim Biophys Acta Gene Regul Mech. 2018. 1861(8):743-751. (IF 5.6)

29. Ma C, Zhang LC, Li JK. Characterization of the complete mitochondrial genome of a bush cricket Xenogryllus marmoratus (Insecta: Orthoptera). Mitochondrial DNA Part B: Resources.

30. Han, B.; Fang, Y.; Feng, M.; Hu, H.; Hao, Y.; Ma, C.; Huo, X.; Meng, L.; Zhang, X.; Wu, F.; Li, J., Brain MembraneProteome and Phosphoproteome Reveal Molecular Basis Associating with Nursing and Foraging Behaviors of Honeybee Workers. J Proteome Res. 2017. 16, (10), 3646-3663.

31. Bezabih G, Cheng H, Han B, Feng M, Xue Y, Hu H，Li J. Phosphoproteome Analysis Reveals Phosphorylation Underpinnings in the Brains of Nurse and Forager Honeybees (Apis mellifera). Sci Rep. 2017.16;7(1):1973.

32. Wang, J.; Zhang, Y.; Qin, X.; Gao, L.; Han, B.; Zhang, D.; Li, J.; Huang, H.; Zhang, W., Efficient expression of an acidic endo-polygalacturonase from Aspergillus niger and its application in juice production. J Agric Food Chem. 2017. 65 (13), 2730-36.

33. Li JY, Cai F, Ye XG, Liang JS, Li JK, Wu MY, Zhao D, Jiang ZD, You ZY, Zhong BX. Comparative Proteomic Analysis of Posterior Silk Glands of Wild and Domesticated Silkworms Reveals Functional Evolution during Domestication. J Proteome Res. 2017. 16(7):2495-2507.

34. Hu H , Bienefeld K , Wegener J , et al. Proteome Analysis of the Hemolymph, Mushroom Body, and Antenna Provides Novel Insight into Honeybee Resistance against Varroa Infestation. Journal of Proteome Research, 2016.. J Proteome Res. 2016. 15, (8), 2841-54.

35. Huo XM,Wu B, Feng M, Han B, Fang Y, Hao Y, Meng LF, Wubie A J,Fan P, Hu H, Qi, Y P, Li J K.Proteomic Analysis Reveals the Molecular Underpinnings of Mandibular Gland Development and Lipid Metabolism in Two Lines of Honeybees (Apis mellifera ligustica). J Proteome Res. 2016. 15, (9), 3342-57.

36. Han Hu, Nan Zheng, Haina Gao, Wenting Dai, Yangdong Zhang, Songli Li, Jiaqi Wang. Immortalized bovine mammary epithelial cells express stem cellmarkers and differentiate in vitro. Cell Biology International. 2016. 40: 861-872.

37.Wu Jiangli , Zhou Chunxue , Wu Pengjie , Xu jin, Guo Yueqin, Xue Fei, Awraris Getachew. Brain metabolomic profiling of eastern honey bee (Apis cerana) infested with the mite Varroa destructor. Plos One, 2017, 12(4):e0175573.

38.Diao Qingyun, Sun Liangxian, Zheng Huajun, Shufa Xu et al. Genomic and transcriptomic analysis of the Asian honeybee Apis cerana provides novel insights into honeybee biology, Scientific Reports，2018, DOI:10.1038/s41598-017-17338-6.https://onlinelibrary.wiley.com/doi/pdf/10.1002/ps.5821

39. Awraris Getachew, Tessema Aynalem Abejew , Jiangli Wu, Jin Xu, Huimin Yu, Jing Tan, Pengjie Wu, Yangyang Tu, Weipeng Kang, Zheng Wang & Shufa Xu, Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis, Scientific Reports, 2020, 10:7532, https://doi.org/10.1038/s41598-020-64022-3

40.Wu Pengjie , Yu Huimin , Xu Jin , et al. Purification of Chinese Sacbrood Virus (CSBV), Gene Cloning and Prokaryotic Expression of its Structural Protein VP1. Molecular Biotechnology, 2018. 10.1007/s12033-018-0121-4.

41.Awraris Getachew, Abebe J. Wubie, Jiangli Wu, Jin Xu, Pengjie Wu, Tessema Aynalem Abejew, Yangyang Tu, Ting Zhou and Shufa Xu. Molecular Identification of Pathogenicity Associated Genes in Honeybee Fungal Pathogen, Ascosphaera apis, by Restricted Enzyme-Mediated Integration (REMI) Constructed Mutants. International Journal of Agriculture and Biology, 2018,20(12): 2879-2890.

42. 段香媛, 武江利, 魏俏红, 冯毛. 2021. 蜂王浆主蛋白2在昆虫细胞-杆状病毒系统中的表达与鉴定. 中国畜牧兽医. 48 (10): 3779-3786.

43. 张丽翠，马川，冯毛，李建科. 基于高分辨质谱和代谢组学技术评估和优化蜂王浆代谢物提取方法. 中国农业科学 2020, 53(18): 3833-3845.

44.李爽，李建科. 浆蜂和意蜂工蜂上颚腺磷酸化蛋白质组分析. 中国农业科学，2017, 24

45.吴鹏杰, 金红岩, 徐进, 武江利, 李雨时, 郭岳琴, 姚军, 徐书法, 吴杰. 中华蜜蜂 SRP9 基因的克隆、序列分析及原核表达. 昆虫学报, 2016, 59(12): 1332-1339

3. 【代表性专利清单】

发明专利

1. 胡菡等，抗MRJP4的配对单克隆抗体、检测MRJP4的ELISA试剂盒和胶体金免疫试纸，中国，专利号ZL202010049812.0

2. 霍新梅：蜂王浆主蛋白1、2、3的快速纯化方法，中国，专利号ZL201910061845.4

3. 胡菡，魏俏红，陈思，冯毛，孟丽峰，韩宾，房宇，马川，李建科；抗蜂王浆主蛋白3的单克隆抗体及检测蜂王浆蛋白3的酶联免疫试剂盒，2020-02-07，中国，ZL201710839199.0.

4. 胡菡等；抗MRJP4的配对单克隆抗体、检测MRJP4的ELISA 试剂盒

和胶体金免疫试纸，2020-11-03，中国，ZL202010049812.0

5. 孟丽峰：一种不开箱自流蜜装置，中国，专利号 ZL 2018 1 1283664.8.

6. 胡菡，永生化奶牛乳腺上皮细胞系及其构建方法和应用；发明专利；发明人：郑楠、胡菡、王加启、高海娜、代文婷；专利号：201410104004.4

7. 一种蜜蜂行为观察器及蜜蜂观测系统ZL201510401633.8发明专利

8. 一种雄蜂卵收集装置ZL201510502738.2发明专利

9. 一种用于观察蜜蜂行为的蜂箱，徐书法，薛菲，国占宝，王品红. ZL 201510247151.1

实用新型专利

1 一种用于蜂箱的蜂脾固定装置ZL201820482243.7

2 一种降温蜂箱ZL201820480639.8

3 一种蜂箱巢门及蜂箱ZL201720758241.1

4 一种用于移虫和取浆的工作台ZL201720834029.9

5 一种蜜蜂自动喂水装置ZL201720816994.3

6 一种新出房蜜蜂收集装置ZL201720740772.8

7 一种半自动蜜蜂取卵装置ZL201820262156.8

8 一种蜜蜂标记装置ZL201820302882.0

9 一种蜂王培育框ZL201820318173.1

10 一种蜜蜂固定装置ZL201820305472.1

11 一种泵蜜装置ZL201820335758.4

12 一种太阳能蜂箱ZL201820302886.9

13一种蜂巢温度监控装置及具有该装置的蜂箱体ZL201820429074.0

14 一种新型蜂箱ZL201721234066.2

15 一种蜜蜂饲喂器ZL201721233533.X

16 食源性土霉素残留快速半定量检测试纸条及试纸卡 ZL 200920110157.4

17 壁挂式自动控制蜜蜂饲喂器的制作方法ZL 200720173596.0

18 蜂蜜压榨机ZL 201720265346.3

19 可分离的蜂箱ZL 201720265425.4

20 便携式蜂箱给药装置ZL 201720654467.7

21 防盗蜂巢门装置ZL 201720655218.X

22 方便饲喂的蜂箱ZL 201720655252.7

23 抽拉式蜂箱ZL 201720655255.0

24 巢础框架ZL 201720655259.9

25 分体可组合式蜂箱ZL 201720656104.7

26 分体设置的蜂箱ZL 201720656525.X

27 用于蜂群防蚂蚁的装置ZL 201720656526.4

28 蜂箱ZL 201721006674.8

30 蜂箱加热装置ZL 201721006681.8

31 蜂箱降温装置ZL 201721006722.3

32 防止盗蜂的装置ZL 201721013503.8

33 蜜蜂轨迹捕捉系统ZL 201721018021.1

34 蜂箱防水保温装置ZL 201721020008.X

35 蜂箱防水装置ZL 201721020255.X

36 蜂箱搬运装ZL 201721020729. 0

4. 【著作清单】

专著

李建科，蜜蜂蛋白质组学研究，中国农业出版社，2016.

李建科，机械化养蜂技术，中原农民出版社，2017.

李建科，话说现代养蜂，中国农业科学技术出版社，2018.

Jianke Li，Agricultural Proteomics Volume 1. Crops, Horticulture, Farm Animals, Food, Insect and Microorganisms，Springer International Publishing AG Switzerland

Jianke Li，Agricultural Proteomics Volume 2，Environmental Stresses，Springer International Publishing AG Switzerland

译著

李建科，蜜蜂生物学，中原农民出版社，2017.

编著

房宇，蜜蜂病虫敌害防控技术，中原农民出版社，2017.

房宇，蜜蜂高效养殖技术，中原农民出版社，2017.

韩宾，蜜蜂文化，中原农民出版社，2017.

韩宾，蜂蜜，中原农民出版社，2017.

孟丽峰，蜂产品知识百问百答，中原农民出版社，2017.

冯毛，中国蜜蜂种质资源，中原农民出版社，2017

胡菡，蜂花粉，中原农民出版社，2017

范沛，蜂王浆，中原农民出版社，2017

张兰，蜂疗，中原农民出版社，2017

吴帆，蜂产品加工技术，中原农民出版社，2017.

张旭凤，蜜蜂高效授粉技术，中原农民出版社，2017.

徐书法，常见蜜粉源植物，中国农业出版社，2021.

5【标准制定】

徐书法，农业行业标准，中华蜜蜂囊状幼虫病诊断技术，NY/T 4043-2021，中华人民共和国农业农村部